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Now, I will start to introduce different functionalities in PaintOmics 4. Pathway analysis is a powerful tool for understanding the biology underlying the data contained in large lists of differentially-expressed genes, metabolites, and proteins resulting from modern high-throughput profiling technologies. The central idea of this approach is to group these long lists of individual features into smaller sets of related biological features (genes and metabolites), usually based on biological processes or cellular components in which genes, proteins, and metabolites are known to be involved.