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Hi all! Welcome the Immunology lab at SASTRA. Let us start with the experiment. Take clean 96 well plate. Label them properly and perform serial dilution using anti-serum. Pipette out 25 ml of saline to each test tube in a row 6 test tubes Add 25 ml of antiserum (A or B) to the first test tube and mix it well. Take 25ml from the first tube and add it to the second tube and then mix it well Then take 25 ml from the second tube repeat the above procedure up to tube 5 Finally take 25 ml from test tube no 5 and discard it [Note: The above procedure results in one fold serial dilution of anti serum] Add 25 ml of 1.5% human RBC suspension to each tube including test tube no. 6 (which serves as control). Mix all tubes well. Close all the tubes with aluminium foil, hold them vertically in the test tube stand and leave it at room temperature for 1 hr. Note down the final dilution of anti serum in each test tube. Examine all the tubes for the occurrence of hemagglutination of RBC. Determine the hemagglutination titer based on the reciprocal of the highest dilution of the antiserum which causes complete agglutination of human RBC. Results Record the hemagglutination titers obtained with a neat diagram indicating hemagglutination reaction at each dilution step.