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Weigh 100mg of agar and dissolve it in 10 ml of 1x Running Buffer. Melt the Agar to Dissolve it properly using a Microwave. Add Antibody to the molten agar. Add it to clean labelled glass plate. Add agar slowly without bubbles. punch holes. Add Standard Known Antigen and perform serial dilution. Add Test antigens to well t1 and t2. And Incubate to observe the results. Have a nice day.