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Objectives: So, in this context, in collaboration with Gratz's group, we evaluated the in vitro antifungal activity of F46 alone and in combination with azoles (voriconazole, posaconazole, fluconazole) against Candida auris, as well as its antibiofilm activity. Finally, we determined the efficacy of these treatments against Candida auris disseminated infection using a murine model. Methodology: To carry out our study we used four strains of Candida auris and two quality control strains. And we evaluated combination of F46 with fluconazole, posaconazole and voriconazole. Synergistic interaction of F46 compounds with azoles was determined by the checkerboard method using this formula to calculate the FIC index and classify the combination in synergy if the FIC index is less than or equal to 0.5, following a conservative approach. Briefly, serial double dilutions of flavone F42 and different azoles were made and distributed in the 96-well microplate in 1:1 volume. In the first column and in the last row, F42 and azoles alone are added respectively to determinate the MIC of these compounds alone. By this method we calculate the reduction of MIC value of one compound when a second compound is add and that allow us to classify the combination as synergy if the FIC index is less than or equal to 0.5, additive, indifferent or antagonism. To assess biofilm disruption by F46, two experiments were carried out. On the one hand, an assay was performed to check the effect of F46 on biofilm formation inhibition by incubating a plate with inoculum and different concentrations of treatment at the same time. On the other hand, another assay was carried out to analyse the effects of F46 on the eradication of preformed biofilm for 24 hours and then adding F46 at different concentrations. Briefly, after incubation at 24h and 48h of treatment application, planktonic cells were discarded, and the plate was washed using sterile phosphate buffered saline (PBS) to remove unbound cells followed by air drying. The plate was then stained with 0.4% of crystal violet solution and washed with sterile water to remove excess stain. And finally, the plate was destained by 95% ethanol solution and measured at 595 nm. Finally, for efficacy assays, OF1 mice were immunosuppressed with cyclophosphamide two days before challenge. All animals were then divided into two groups and each group was infected with JCM-15448 or FMR-17045 intravenously and the following day they were grouped into groups of 13 animals according to treatment: anidulafungin, posaconazole, flavone F46 and the combination posaconazole+F46, plus a control group receiving saline. Therapies were initiated one-day post-infection and administered daily for 10 days. Efficacy was evaluated by survival studies up to 23 days post infection. Tissue burden was analysed in kidneys, lungs, and liver during treatment on day 8 post-infection, and after treatment on day 23 post-infection. Next, I am going to explain results we have obtained.