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At the seventieth hour 50 microliters of colcemid, a synthetic alkaloid derived from colchicine, extracted from the autumn Colchicum, are added. It is used to stop cells in metaphase. It prevents the formation of the spindle during mitosis, depolymerizing the microtubules, thus causing halt in the metaphase. Work continues under an organic hood so as not to contaminate the crops. The tubes are put back in a thermostat at 37 ° C until the end of the growth of the crops always on the rotating plate