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So, in this context, our group in collaboration with a team from the University of Graz in Austria, we want to identify flavones compounds with antifungal potential in combination with standard antifungals drugs, specifically the azoles. To carry out our goal, we first analyzed the effect of these compounds in vitro, using the Checkerboard assay with different fungal genera to determine the best candidate for antifungal combinations. And then later, animal experiments will be carried out. In this seminar I am going to show results we have obtained with the compound 3,6 dihydroxyflavone, abbreviated as F42. Therefore, the objective of this present experiment is to determine in vitro antifungal activity of F42 alone or in combination with the azoles against clinically relevant fungi. To determine interaction of F42 compounds with azoles, checkerboard assay based on CLSI microdilution technic were performed. Briefly, F42 and different azoles are diluted in series, using a dilution of two-factor and are distributed in a 96-well microplate and added in 1:1 volume. In the first column and last row, we are added F42 and azoles alone respectively. The data obtained were analyzed using the model-fractional inhibitory concentration index (FICI): An FICI value of ≤0.5 was interpreted as synergy whereas the FICI values between 0.5 and 1.0 were interpreted as additive. FICI values >4.0 were considered as antagonism and FICI values between 1.0 and 4.0 were considered as indifferent, following a conservative approach.